Čeština
EnglishLaboratory of Virology
Research areas of the laboratory:
Understanding and management of plant viral diseases
· Study of molecular properties of plant viruses by immunological and molecular biological methods
· Genome mapping of plant viruses, viral isolates and phylogenetic analysis
· Preparation of polyclonal antibodies against recombinant viral structural and nonstructural proteins for investigation of virus life-cycle
· Virus detection using antibodies- and/or DNA/RNA-based methods (LAMP, RT-LAMP, PCR, RT-PCR, qPCR)
Biotechnological utilization of plant viruses
· Design of plant viral vectors based on several RNA and DNA plant viruses
· Adaptation of plant viral vectors to GoldenBraid cloning system
· Transient expression of heterologous proteins in plants using vectors derived from plant viruses
· Peptide/epitope display on the surface of virus particles (electron microscopy)
Metabolic engineering of plant cells
· Development of platform for expression and accumulation of authentic human glycoproteins such as antibodies in plants
· Plant genome editing (Crispr, RNAi)
· Protein expression using plant tissue cultures
Influence of abiotic stress on plant virus infection
Analysis of virus infection upon heat, drought and cold treatment of plants
Workers
- Jan Fousek
- Ing. Jiban Kumar Ph.D.
- Bc. Radek Vítek
head of the laboratoryscientist
- Mgr. Tomáš Moravec PhD
deputy head of the laboratorysenior scientist
- Doc. RNDr. Noemi Čeřovská CSc.
scientist
- Mgr. Hana Hoffmeisterová Ph.D.
- Dr.rer.nat.Ing. Helena Plchová
scientific assistant
- RNDr. Oldřich Navrátil CSc.
graduated technical assistant
- ing. Jitka Svobodová
postgraduate student
- Ing. Jakub Dušek
technician
Research projects
- Enhancement of Cannabis sativa in vitro regeneration after transformation with Agrobacterium tumefaciens , GA AV , Tomáš Moravec
- Nanomaterials based on Tobacco mosaic virus , MŠMT , Tomáš Moravec
- Efektivní metody diagnostiky tobamovirů a tospovirů, zlepšení metody ochrany a zvýšení odolnosti rajčat proti těmto patogenům , TA ČR , Tomáš Moravec
- Genome editing in plants - IEB CAS contribution , MŠMT , Tomáš Moravec
- Impact of humanised glycosylation pathway on protein accumulation and traficking in plant seeds , GA ČR , Tomáš Moravec
- Impact of different topology of heterologous antigenic determinants in PVX-based plant viral vector on their expression level and immunogenicity , GA ČR , Noemi Čeřovská
- Biosafe plant virus expression system for transient production of human papillomavirus oncoproteins and their use for therapeutic vaccine development , GA ČR , Noemi Čeřovská
- Variability of Potato leafroll luteovirus (PLRV), improved reliability of its detection and utilization of transgenic resistance , MZE NAZV , Tomáš Moravec
- Genomics and proteomics in plant breeding , MŠMT , Noemi Čeřovská
Publications
2009
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 10 3583-3598 2009 Fulltext: PDF link on intranet only
- PHOTOSYNTHETICA 47 409-414 2009
- BIOLOGIA PLANTARUM 53 691-696 2009
- BIOLOGIA PLANTARUM 53 475-483 2009
2008
- PROTEIN EXPRESSION AND PURIFICATION 58 154-161 2008 Fulltext: PDF link on intranet only
- BIOLOGIA PLANTARUM 52 184-186 2008 Fulltext: PDF link on intranet only
- PLANT CELL, TISSUE AND ORGAN CULTURE 94 261-267 2008 Fulltext: PDF link on intranet only
- FOLIA MICROBIOLOGICA 53 438-442 2008 Fulltext: PDF link on intranet only
- CELL BIOCHEMISTRY AND FUNCTION 26 582-590 2008 Fulltext: PDF link on intranet only
- VĚDECKÉ PRÁCE - VÝZKUMNÝ ÚSTAV BRAMBORÁŘSKÝ HAVLÍČKůV BROD 16 57-64 2008 Fulltext: PDF link on intranet only