Čeština
EnglishAnalytical Methods for Brassinosteroid Analysis: Recent Advances and Applications
Oklestkova J., Kvasnica M., Strnad M.,
PLANT AND CELL PHYSIOLOGY 65: 1618–1626, 2024
Klíčová slova: Brassinosteroids, chromatography, mass spectrometry, immunoassays, quantification, chemical synthesis
Abstrakt: Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages, and limitations of various analytical techniques, including immunoassays, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) that are used for the detection and quantification of BRs from complex plant matrices. We also explore the use of isotopically labeled internal standards to improve the accuracy and reliability of BR quantification.
DOI: 10.1093/pcp/pcae038
Autoři z ÚEB: Miroslav Kvasnica, Jana Oklešťková, Miroslav Strnad
PLANT AND CELL PHYSIOLOGY 65: 1618–1626, 2024
Klíčová slova: Brassinosteroids, chromatography, mass spectrometry, immunoassays, quantification, chemical synthesis
Abstrakt: Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages, and limitations of various analytical techniques, including immunoassays, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) that are used for the detection and quantification of BRs from complex plant matrices. We also explore the use of isotopically labeled internal standards to improve the accuracy and reliability of BR quantification.
DOI: 10.1093/pcp/pcae038