Čeština
EnglishEffect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots
Cvikrová, Milena; Binarová, Pavla; Cenklová, Věra; Eder, Josef; Doležel, Jaroslav; Macháčková, Ivana
PLANT SCIENCE 164 [5]: 823-832, 2003
Klíčová slova: cell cycle; polyamines
Abstrakt: 2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to the inhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescine conjugates in cells after AIP-treatment might represent stress response in cells after AIP application. Increased PA conjugation suggests that it has an important role in the control of free PA contents in cells.
DOI:
Autoři z ÚEB: Milena Cvikrová, Jaroslav Doležel
PLANT SCIENCE 164 [5]: 823-832, 2003
Klíčová slova: cell cycle; polyamines
Abstrakt: 2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to the inhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescine conjugates in cells after AIP-treatment might represent stress response in cells after AIP application. Increased PA conjugation suggests that it has an important role in the control of free PA contents in cells.
DOI: