Čeština
EnglishFlow cytometric analysis of variation in the level of nuclear DNA endoreduplication in the cotyledons amongst Vigna radiata cultivars
Pal, A.; Vrána, Jan; Doležel, Jaroslav
CARYOLOGIA 57 [3]: 262-266, 2004
Klíčová slova: Cotyledon; endoreduplication; flow cytometry
Abstrakt: Most of the differentiated plant cells replicate their nuclear DNA but there is no chromosome condensation, strand separation, nor cytokinesis, resulting in multiple copies of the genome, and the phenomenon is called endoreduplication. In this communication we report the distribution pattern of DNA endoreduplication in the nuclei of the mature cotyledons of six cultivars of Vigna radiata (mungbean). It has shown that the maximum ploidy level present in the cotyledonary tissues of V radiata is 64C, where 2C is DNA amount of unreplicated diploid nucleus, except in the cultivar ML 337, where only up to 32C DNA content was noted. Considerable difference in the level of C-value distribution amongst different cultivars of V radiata has been found in the present investigation, This is the first report on flow cytometric analysis showing variation in the level of nuclear endoreduplication amongst the cultivars of a grain legume.
DOI:
Autoři z ÚEB: Jaroslav Doležel
CARYOLOGIA 57 [3]: 262-266, 2004
Klíčová slova: Cotyledon; endoreduplication; flow cytometry
Abstrakt: Most of the differentiated plant cells replicate their nuclear DNA but there is no chromosome condensation, strand separation, nor cytokinesis, resulting in multiple copies of the genome, and the phenomenon is called endoreduplication. In this communication we report the distribution pattern of DNA endoreduplication in the nuclei of the mature cotyledons of six cultivars of Vigna radiata (mungbean). It has shown that the maximum ploidy level present in the cotyledonary tissues of V radiata is 64C, where 2C is DNA amount of unreplicated diploid nucleus, except in the cultivar ML 337, where only up to 32C DNA content was noted. Considerable difference in the level of C-value distribution amongst different cultivars of V radiata has been found in the present investigation, This is the first report on flow cytometric analysis showing variation in the level of nuclear endoreduplication amongst the cultivars of a grain legume.
DOI: