Čeština
EnglishGeneration of deletion lines in allohexaploid bread wheat
Serra S., Svačina R., Bartoš J., Sourdille P.
Plant Gametogenesis. Methods in Molecular Biology. 2484: 189-199, 2022
Klíčová slova: Wheat, Triticum aestivum, Deletion line, Gametocidal, Homeologs
Abstrakt: Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopolyploidy. One way to face this challenge is to decrease the size of the interval bearing the gene of interest both genetically and physically, in order to reduce significantly the number of potential candidate genes. In this chapter, we describe a technical approach to produce chromosome-specific deletion lines to locate precisely genes of interest onto wheat chromosomes, a step forward to their cloning.
DOI: 10.1007/978-1-0716-2253-7_14
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš, Radim Svačina
Plant Gametogenesis. Methods in Molecular Biology. 2484: 189-199, 2022
Klíčová slova: Wheat, Triticum aestivum, Deletion line, Gametocidal, Homeologs
Abstrakt: Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopolyploidy. One way to face this challenge is to decrease the size of the interval bearing the gene of interest both genetically and physically, in order to reduce significantly the number of potential candidate genes. In this chapter, we describe a technical approach to produce chromosome-specific deletion lines to locate precisely genes of interest onto wheat chromosomes, a step forward to their cloning.
DOI: 10.1007/978-1-0716-2253-7_14
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš, Radim Svačina