Tomaštíková E.D., Vaculíková J., Štenclová V., Kaduchová K., Pobořilová Z., Paleček J.J., Pečinka A.
PLANT JOURNAL 119: 1418-1432, 2024

Klíčová slova: DNA damage repair, replicative stress, DNA–protein crosslink, RTEL1, TEBICHI, SMC5/6 complex, genome stability, Arabidopsis thaliana
Abstrakt: DNA–protein crosslinks (DPCs) are highly toxic DNA lesions represented by proteins covalently bound to the DNA. Persisting DPCs interfere with fundamental genetic processes such as DNA replication and transcription. Cytidine analog zebularine (ZEB) has been shown to crosslink DNA METHYLTRANSFERASE1 (MET1). Recently, we uncovered a critical role of the SMC5/6-mediated SUMOylation in the repair of DPCs. In an ongoing genetic screen, we identified two additional candidates, HYPERSENSITIVE TO ZEBULARINE 2 and 3, that were mapped to REGULATOR OF TELOMERE ELONGATION 1 (RTEL1) and polymerase TEBICHI (TEB), respectively. By monitoring the growth of hze2 and hze3 plants in response to zebularine, we show the importance of homologous recombination (HR) factor RTEL1 and microhomology-mediated end-joining (MMEJ) polymerase TEB in the repair of MET1-DPCs. Moreover, genetic interaction and sensitivity assays showed the interdependency of SMC5/6 complex, HR, and MMEJ in the homology-directed repair of MET1-DPCs in Arabidopsis. Altogether, we provide evidence that MET1-DPC repair in plants is more complex than originally expected.
DOI: 10.1111/tpj.16863
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Kateřina Kaduchová, Aleš Pečinka, Zuzana Pobořilová, Eva Dvořák Tomašt...