Čeština
EnglishIsolation of pathogenesis-related proteins from TMV-Infected tobacco and their influence on infectivity of TMV
Šindelářová, Milada; Šindelář, Luděk
PLANT PROTECTION SCIENCE 41: 52-57, 2005
Klíčová slova: Nicotiana tabacum; PR-proteins; PAGE
Abstrakt: The composition of pathogenesis-related proteins (PR-proteins) and inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15–16 kDa (Group 1), 27–28 kDa (Group 3: chitinases) and 36–40 kDa (Group 2a: β-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.
DOI:
Autoři z ÚEB:
PLANT PROTECTION SCIENCE 41: 52-57, 2005
Klíčová slova: Nicotiana tabacum; PR-proteins; PAGE
Abstrakt: The composition of pathogenesis-related proteins (PR-proteins) and inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15–16 kDa (Group 1), 27–28 kDa (Group 3: chitinases) and 36–40 kDa (Group 2a: β-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.
DOI: