Čeština
EnglishPotato transformation by T-DNA cytokinin gene
Ondřej M., Macháčková I., Čatský J., Eder J., Hrouda M., Pospíšilová J., Synková H.
BIOLOGIA PLANTARUM 32: 401-406, 1990
Klíčová slova: transgenic plants, cytokinins, photosynthetic rate, chlotrophyll content, Solanum tuberosum
Abstrakt: Abstract. Potato plants were transformed by the A. tumeEaciens vector which integrates into the plant genome two foreign genes only: pTi C58 T-DNA gene 4 Opt) responsible for elevated synthesis of cytokinins and kanamycin resistance gene. Three teratoma clones studied showed approximately 3 times, 6 times and 9 times increased levels of zeatin riboside and zeatin in comparison with untraasformed controls and slight increase of the IAA level. Shoots formed roots in agar cultures sporadically, if the increase of zeatin riboside and zeatin levels was not higher than 6 times the control level. The chlorophyll content and net photosynthetic rate decreased with increasing levels of zeatin and zeatin riboside.
DOI: 10.1007/BF02890884
Autoři z ÚEB: Helena Synková
BIOLOGIA PLANTARUM 32: 401-406, 1990
Klíčová slova: transgenic plants, cytokinins, photosynthetic rate, chlotrophyll content, Solanum tuberosum
Abstrakt: Abstract. Potato plants were transformed by the A. tumeEaciens vector which integrates into the plant genome two foreign genes only: pTi C58 T-DNA gene 4 Opt) responsible for elevated synthesis of cytokinins and kanamycin resistance gene. Three teratoma clones studied showed approximately 3 times, 6 times and 9 times increased levels of zeatin riboside and zeatin in comparison with untraasformed controls and slight increase of the IAA level. Shoots formed roots in agar cultures sporadically, if the increase of zeatin riboside and zeatin levels was not higher than 6 times the control level. The chlorophyll content and net photosynthetic rate decreased with increasing levels of zeatin and zeatin riboside.
DOI: 10.1007/BF02890884