CYTOMETRY

Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants

Temsch E.M., Koutecký P., Urfus T., Šmarda P., Doležel J.
CYTOMETRY 101 : 710-724 , 2022
Keywords: best practices, C-value, flow cytometry, GC content, genome size, plant sciences, plant standard species, standardization

Chromosome analysis and sorting

Doležel J., Lucretti S., Molnár I., Cápal P., Giorgi D.
CYTOMETRY 99 : 328-342 , 2021
Keywords: Cell cycle synchronization, mitotic metaphase chromosomes, liquid chromosome suspension, DNA isolation, DNA amplification, gene mapping and cloning, genome sequencing, marker development, repetitive DNA labelling

Best practices in plant cytometry

Galbraith, D., Loureiro, J., Antoniadi, I., Bainard, J., Bureš, P., Cápal, P., Castro, M., Castro, S., Čertner, M., Čertnerová, D., Chumová, Z., Doležel, J., Giorgi, D., Husband, B.C, Kolář, F., Koutecký, P., Kron, P., Leitch, I.J., Ljung, K., Lopes, S., Lučanová, M., Lucretti, S., Ma, W., Melzer, S., Molnár, I., Novák, O., Poulton, N., Skalický, V., Sliwinska, E., Šmarda, P., Smith, T.W., Sun, G., Talhinhas, P., Tárnok, A., Temsch, E.M., Trávníček, P., Urfus, T.
CYTOMETRY 99 : 311-317 , 2021
Keywords: best practices, chromosome analysis, cytometry, nuclear suspensions, plant sciences, protoplasts and organelle analysis, single cell suspensions

Challenges of flow-cytometric estimation of nuclear genome size in Orchids, a plant group with both whole-genome and progressively partial endoreplication

Trávníček, P., Ponert, J., Urfus, T., Jersáková, J., Vrána, J., Hřibová, E., Doležel, J., Suda, J.
CYTOMETRY 87A : 958-966 , 2015
Keywords: C-value; endopolyploidy; flow cytometry; genome size; histogram interpretation; nuclear DNA content; orchid; progressively partial endoreplication

Nuclear genome size: are we getting closer?

Doležel, J., Greilhuber, J.
CYTOMETRY 77A : 635-642 , 2010
Keywords: cytometric techniques; reference standards; genome sequencing; conversion factor; nuclear DNA content; C-value

Plant flow cytometry - far beyond the stone age.

Loureiro, J., Doležel, J., Grielhuber, J., Santos, C., Suda, J.
CYTOMETRY 73A : 579-580 , 2008
Keywords:

Nuclear DNA content and genome size of trout and human

Doležel, Jaroslav; Bartoš, Jan; Voglmayr, H.; Greilhuber, J.
CYTOMETRY 51 : 127-128 , 2003
Keywords: flow cytometry; nuclear DNA content; genome size