Plant Cytogenetics: Methods and Protocols

Construction of BAC libraries from flow-sorted chromosomes

Šafář, J., Šimková, H., Doležel, J.
Plant Cytogenetics: Methods and Protocols : 135-149 , 2016
Keywords: BAC library , BAC vector , Chromosomes , DNA cloning , High molecular weight (HMW) DNA , Pulse-fi eld gel electrophoresis (PFGE) , Clone

Flow sorting plant chromosomes

Vrána, J., Cápal, P., Číhalíková, J., Kubaláková, M., Doležel, J.
Plant Cytogenetics: Methods and Protocols : 119-134 , 2016
Keywords: Cell cycle synchronization , Chromosome isolation , Cytogenetic stocks , FISH , FISHIS , Flow cytometry and sorting , Metaphase accumulation , Plants

Localization of low-copy DNA sequences on mitotic chromosomes by FISH

Karafiátová, M., Bartoš, J., Doležel, J.
Plant Cytogenetics: Methods and Protocols : 49-64 , 2016
Keywords: Cell cycle synchronization , Cytogenetic mapping , Fluorochrome , Low-copy probe , Mitotic metaphase chromosomes , Probe purifi cation , Post-fi xation , Quantum yield