Čeština
EnglishChromosomal allocation of DNA sequences in wheat using flow-sorted chromosomes
Cápal, P., Vrána, J., Kubaláková, M., Endo, T.R., Doležel, J.
Chromosome and Genomic Engineering in Plants: Methods and Protocols : 157-170, 2016
Keywords: Chromosomal allocation , Transgene , Flow cytometry , Chromosome sorting , Single chromosome amplifi cation , Chromosome genomics , FISH , Wheat
Abstract: Flow cytometry enables chromosomes to be sorted into different groups based on their characteristics, such as relative DNA content and the presence of repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat using fl ow-sorted chromosomes combined with fl uorescence in situ hybridization and PCR analyses.
DOI: 10.1007/978-1-4939-4931-1_12
Fulltext: contact IEB authors
IEB authors: Petr Cápal, Jaroslav Doležel, Jan Vrána
Chromosome and Genomic Engineering in Plants: Methods and Protocols : 157-170, 2016
Keywords: Chromosomal allocation , Transgene , Flow cytometry , Chromosome sorting , Single chromosome amplifi cation , Chromosome genomics , FISH , Wheat
Abstract: Flow cytometry enables chromosomes to be sorted into different groups based on their characteristics, such as relative DNA content and the presence of repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat using fl ow-sorted chromosomes combined with fl uorescence in situ hybridization and PCR analyses.
DOI: 10.1007/978-1-4939-4931-1_12
Fulltext: contact IEB authors
IEB authors: Petr Cápal, Jaroslav Doležel, Jan Vrána