Doležel, J., Šimková, H., Kubaláková, M., Šafář, J., Suchánková, P., Číhalíková, J., Bartoš, J., Valárik, M.
In: Feuillet, C., Muehlbauer, G.J. (eds.): Genetics and Genomics of the Triticeae. Springer, New York. : 285-316, 2009

Keywords: Wheat, Triticeae, physical mapping, chromosomes, flow-sorting, BAC libraries
Abstract: The Triticeae species are unique among the important agricultural crops in possessing massive genomes with a prevalence of dispersed DNA repeats. The highest level of complexity is observed in tetraploid and hexaploid wheat whose nuclear genomes comprise two and three homoeologous genomes, respectively. Polyploidy and the presence of repeats make gene cloning and genome sequencing in the Triticeae extremely difficult. Chromosome genomics simplifies these tasks by targeting single chromosomes and chromosome arms, which represent only a few percent of the nuclear genomes. The advantages of this strategy over a whole-genome approach include the avoidance of problems due to the presence of homoeologs in wheat, reduction of work to manageable portions, cost efficiency, and an opportunity to structure collaborative projects where individual laboratories work on particular chromosomes. In this chapter, we describe how chromosomes and chromosome arms can be isolated by flow cytometric sorting and we review development of flow cytogenetics in the Triticeae. We then discuss various applications of flow-sorted chromosomes and assess the potential of chromosome genomics in the Triticeae.
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IEB authors: Jan Bartoš, Jaroslav Doležel, Jan Šafář, Hana Šimková, Pavla Suchánková, Miroslav Valárik