Čeština
EnglishCot-based cloning and sequencing of the short arm of wheat chromosome 1B
Šimková, H., Janda, J., Hřibová, E., Šafář, J., Doležel, J.
PLANT, SOIL AND ENVIRONMENT 53 [10]: 437-441, 2007
Keywords: Cot; BAC library; wheat
Abstract: Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches – i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library – was tested in this work.
DOI:
IEB authors: Jaroslav Doležel, Eva Hřibová, Jan Šafář, Hana Šimková
PLANT, SOIL AND ENVIRONMENT 53 [10]: 437-441, 2007
Keywords: Cot; BAC library; wheat
Abstract: Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches – i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library – was tested in this work.
DOI: