Kobayashi, F., Katagiri, S., Karasawa, W., Hanawa, Y., Kanamori, H., Ito, Y., Fujisawa, H., Mukai, Y., Tanaka, T., Kaneko, S., Watanabe, S., Sakaguchi, T., Nasuda, S., Hayakawa, K., Abe, C., Ohno, R., Iehisa, J.C.M., Takumi, S., Doležel, J., Ogihara, Y., Matsumoto, T., Katayose, Y., Wu, J., Handa, H.
Advances in wheat genetics: From genome to field : 101-107, 2015

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Abstract: The International Wheat Genome Sequencing Consortium (IWGSC) has been promoting the decoding of the wheat genome sequence to enhance our knowledge of the structure and function of the wheat genome. The vision of the consortium is to establish a high quality reference sequence with a strategy to sequence its whole genome using BAC (bacterial artifi cial chromosome) clones aligned on the physical map of each chromosome. Under the framework of IWGSC, we are in charge of the chromosome 6B with the fi nancial support from the Japanese Ministry of Agriculture, Forestry and Fisheries (“Genomics for Agricultural Innovation” project and “Genomics-based Technology for Agricultural Improvement” project) and Nisshin Flour Milling Inc. The “KomugiGSP” (Genome Sequence Program) ( http://komugigsp. dna.affrc.go.jp/index.html ) currently consists of the following four main research subjects: (1) Construction of 6B-specifi c BAC library and development of DNA markers, (2) comprehensive analysis of transcripts for gene annotation, (3) construction of the BAC physical map and genome sequencing (BAC-by-BAC and whole chromosome survey sequencing), and (4) development of annotation pipeline. Here, we report our current progresses on the construction of a physical map for chromosome 6B using chromosome arms-specifi c BAC libraries with DNA markers and BAC-based genomic sequencing.
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IEB authors: Jaroslav Doležel