Čeština
EnglishEasyClick: an improved system for confocal microscopy of live roots with a user optimized sample holder
Kaduchová, K., Čmiel, V., Koláčková, V., Pecinka, A.
PLANTA 259: 22, 2024
Keywords: Barley, Confocal microscopy, EasyClick, Growth, Hordeum vulgare, Live cell imaging, Plant, Root
Abstract: Preparation and mounting of the samples are key factors for successful live cell microscopy. To acquire biologically relevant data, it is necessary to minimize stress and avoid physical damage to plant tissues during the installation of the sample into the microscope. This is challenging, particularly when the whole plant is mounted as the living sample needs to be properly anchored in the microscopic system to obtain high-quality and high-resolution data. Here, we present a useroptimized sample holder EasyClick for live cell inverted confocal microscopic analysis of plant roots with diameters from 0.3 to 0.7 mm. The EasyClick holder was tested on an inverted confocal microscope using germinating plants of several cereals. Nevertheless, it can be directly used on other types of inverted microscopes from various producers and on diferent plant species. The EasyClick holder efectively restricts root lateral and vertical movements. This greatly improves the conditions for time-lapse microscopy of the samples of interest.
DOI: 10.1007/s00425-023-04293-y
Fulltext: contact IEB authors
IEB authors: Kateřina Kaduchová, Veronika Koláčková, Aleš Pečinka
PLANTA 259: 22, 2024
Keywords: Barley, Confocal microscopy, EasyClick, Growth, Hordeum vulgare, Live cell imaging, Plant, Root
Abstract: Preparation and mounting of the samples are key factors for successful live cell microscopy. To acquire biologically relevant data, it is necessary to minimize stress and avoid physical damage to plant tissues during the installation of the sample into the microscope. This is challenging, particularly when the whole plant is mounted as the living sample needs to be properly anchored in the microscopic system to obtain high-quality and high-resolution data. Here, we present a useroptimized sample holder EasyClick for live cell inverted confocal microscopic analysis of plant roots with diameters from 0.3 to 0.7 mm. The EasyClick holder was tested on an inverted confocal microscope using germinating plants of several cereals. Nevertheless, it can be directly used on other types of inverted microscopes from various producers and on diferent plant species. The EasyClick holder efectively restricts root lateral and vertical movements. This greatly improves the conditions for time-lapse microscopy of the samples of interest.
DOI: 10.1007/s00425-023-04293-y
Fulltext: contact IEB authors
IEB authors: Kateřina Kaduchová, Veronika Koláčková, Aleš Pečinka