Čeština
EnglishThe effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba
Šafářová, D.; Kopecký, David; Vagera, Jiří
BIOLOGIA PLANTARUM 49 [2]: 261-264, 2005
Keywords: campion; green regenerants; ploidy analysis
Abstract: The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shock treatments (33 °C, and 37 °C) was applied for 1, 3, and 5 d. Across all media compositions tested, the best androgenic response was observed at 25 °C and after a one-day treatment at 33 °C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm-3) and sucrose. The green, albino and chimeric, only female, plants were regenerated. Flow cytometry of 110 regenerants identified mixoploids (n+2n and 2n+4n) and dihaploids.
DOI:
IEB authors: David Kopecky
BIOLOGIA PLANTARUM 49 [2]: 261-264, 2005
Keywords: campion; green regenerants; ploidy analysis
Abstract: The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shock treatments (33 °C, and 37 °C) was applied for 1, 3, and 5 d. Across all media compositions tested, the best androgenic response was observed at 25 °C and after a one-day treatment at 33 °C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm-3) and sucrose. The green, albino and chimeric, only female, plants were regenerated. Flow cytometry of 110 regenerants identified mixoploids (n+2n and 2n+4n) and dihaploids.
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