Čeština
EnglishFlow cytogenetics
Doležel, Jaroslav; Kubaláková, Marie; Vrána, Jan; Bartoš, Jan
In Encyclopedia of Plant and Crop Science. New York : www.dekker.com : 460-463, 2004
Keywords: flow cytogenetics; flow cytometry; chromosome sorting
Abstract: Flow cytogenetics uses flow cytometry and sorting to classify and purify isolated mitotic chromosomes. The chromosomes are analyzed while moving at high speed in a liquid stream. This gives high resolution and statistical precision, because large populations of chromosomes can be analyzed in a short time. Flow sorting allows purification of large quantities of specific chromosomes according to their fluorescence, which can be used for physical genome mapping, production of recombinant DNA libraries, and protein analysis. Chromosome classification by flow cytometry (flow karyotyping) may be used to detect structural and numerical chromosome changes.
DOI:
IEB authors: Jan Bartoš, Jaroslav Doležel
In Encyclopedia of Plant and Crop Science. New York : www.dekker.com : 460-463, 2004
Keywords: flow cytogenetics; flow cytometry; chromosome sorting
Abstract: Flow cytogenetics uses flow cytometry and sorting to classify and purify isolated mitotic chromosomes. The chromosomes are analyzed while moving at high speed in a liquid stream. This gives high resolution and statistical precision, because large populations of chromosomes can be analyzed in a short time. Flow sorting allows purification of large quantities of specific chromosomes according to their fluorescence, which can be used for physical genome mapping, production of recombinant DNA libraries, and protein analysis. Chromosome classification by flow cytometry (flow karyotyping) may be used to detect structural and numerical chromosome changes.
DOI: