Doležel J., Urbiš P., Said M., Lucretti S., Molnár I.
NUCLEUS 66: 355-369, 2023

Keywords: flow cytometry, chromosome sorting, genome sequencing, gene cloning, physical mapping
Abstract: Flow cytogenetics is a high-throughput technique that classifes large populations of mitotic chromosomes according to their fuorescence and light scattering as they move in a single fle rapidly in a narrow stream of liquid. Flow karyotyping can detect structural and numerical chromosome changes, and chromosomes purifed by sorting have been used to discover the three-dimensional organization of DNA, characterize the proteome, and describe the organization of the perichromosomal layer at the nanometre level. However, the most frequent and important use of fow cytogenetics has been the isolation of diferent chromosomes. Chromosome sorting has made it possible to dissect nuclear genomes into small and defned parts, therefore allowing targeted, simplifed, and more economical genomic analyses. The uses of fow-sorted chromosomes for genome complexity reduction and targeted analysis have expanded hand-in-hand with the progress of molecular biology and genomics techniques. These uses include the targeted development of DNA markers, the construction of chromosomespecifc DNA libraries, physical mapping, draft genome sequencing, and gene cloning.
DOI: 10.1007/s13237-023-00450-6
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IEB authors: Jaroslav Doležel, Mahmoud Said, Petr Urbiš