Čeština
EnglishFlow cytometry and cell sorting in plant biotechology
Galbraith, D. W.; Bartoš, Jan; Doležel, Jaroslav
In Flow Cytometry for Biotechnology. Oxford : Oxford University Press : 291-322, 2005
Keywords: Flow cytometry; cell sorting; plant biotechnology
Abstract: Flow cytometry and cell sorting has been applied to a wide variety of different problems in plant biotechnology and genomics. Applications of flow cytometry and sorting have been particularly boosted by the development of methods for expression of intrinsically fluorescent markers within transgenic organisms, of which the Green Fluorescent Protein and its derivatives, are the most popular and important. Flow cytometric analysis and sorting applications have also been developed for use with subcellular organelles, including nuclei, mitochondria, and chloroplasts, based either on intrinsic fluorescence (chloroplasts), in vivo GFP expression and targeting, or staining with particular fluorochromes.
DOI:
IEB authors: Jan Bartoš, Jaroslav Doležel
In Flow Cytometry for Biotechnology. Oxford : Oxford University Press : 291-322, 2005
Keywords: Flow cytometry; cell sorting; plant biotechnology
Abstract: Flow cytometry and cell sorting has been applied to a wide variety of different problems in plant biotechnology and genomics. Applications of flow cytometry and sorting have been particularly boosted by the development of methods for expression of intrinsically fluorescent markers within transgenic organisms, of which the Green Fluorescent Protein and its derivatives, are the most popular and important. Flow cytometric analysis and sorting applications have also been developed for use with subcellular organelles, including nuclei, mitochondria, and chloroplasts, based either on intrinsic fluorescence (chloroplasts), in vivo GFP expression and targeting, or staining with particular fluorochromes.
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