Čeština
EnglishIsolation of chromosomes from Picea abies L. and their analysis by flow cytometry
Überall, I.; Vrána, Jan; Bartoš, Jan; Šmerda, J.; Doležel, Jaroslav; Havel, L.
BIOLOGIA PLANTARUM 48 [2]: 199-203, 2004
Keywords: Vicia faba; composite map
Abstract: A high-yield method for preparation of suspensionsof intact Norway spruce [Picea abies (L.) Karst] chromosomes was developed for the first time. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatments with 0.625 mM hydroxyurea for 18 h and after 8 h recovery in distilled water with 0.05
DOI:
IEB authors: Jan Bartoš, Jaroslav Doležel
BIOLOGIA PLANTARUM 48 [2]: 199-203, 2004
Keywords: Vicia faba; composite map
Abstract: A high-yield method for preparation of suspensionsof intact Norway spruce [Picea abies (L.) Karst] chromosomes was developed for the first time. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatments with 0.625 mM hydroxyurea for 18 h and after 8 h recovery in distilled water with 0.05
DOI: