Post-irradiation changes in antioxidant activity of spindle tree leaves from different areas

Kim J.K., Cha M., Wilhelmová N., Mukherjee A.
KOREAN JOURNAL OF ENVIRONMENTAL BIOLOGY 29: 280-285, 2011

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Abstract: Plants can be a good indicator which reflects the level of environmental pollution. In this study, spindle trees (Euonymus japonica) were collected from a clean area (Kijang) as well as a heavily polluted area (Onsan) to check applicability of irradiation combined with plant bioassay to environmental monitoring. The leaves were irradiated with 0, 50 and 100 Gy of gamma rays, and then evaluated for antioxidative capacity with 1,1- diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) analysis. The result shows that there was no significant changes in SOD and EDA (Electron Donationg Ability) in the samples collected from a polluted area. In the meanwhile, SOD increased in the samples from a clean area until 6 to 10 hours after irradiation, then it decreased gradually until 24 hours after irradiation. Plants in the polluted area have developed higher resistance to oxidative stress induced by ionizing radiation than those in the relatively clean area.
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IEB authors: Naďa Wilhelmová