Čeština
EnglishPurification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography
Dobrev, Petre; Havlíček, Libor; Vágner, Martin; Malbeck, Jiří; Kamínek, Miroslav
JOURNAL OF CHROMATOGRAPHY 1075: 159-166, 2005
Keywords: Auxin; Abscisic acid; Solid phase extraction
Abstract: A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in “heart cutting” mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
DOI:
IEB authors: Petre I. Dobrev, Libor Havlíček, Miroslav Kamínek, Jiří Malbeck, Martin Vágner
JOURNAL OF CHROMATOGRAPHY 1075: 159-166, 2005
Keywords: Auxin; Abscisic acid; Solid phase extraction
Abstract: A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in “heart cutting” mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.
DOI: