Čeřovská, Noemi; Pečenková, Tamara; Filigarová, Marie; Dědič, P.
FOLIA MICROBIOLOGICA 52 [1]: 61-64, 2007

Keywords: SPONGOSPORA-SUBTERRANEA; NUCLEOTIDE-SEQUENCE; GENOME ORGANIZATION
Abstract: The nucleotide sequence was determined for Czech potato mop-top virus (PMTV) isolate Korneta-Nemilkov, found in the potato field situated in South Bohemia. The nucleotide and amino acid sequences were compared with other PMTV isolates available in databases. The sequence identity was always > 99 % when Czech isolate RNA 2 and RNA 3 sequences were compared with each of the 3 Danish isolates and with Sw isolate, and slightly lower when compared to Scottish isolates. Similarity of deduced proteins was 100 % for 5 out of 6 proteins used in comparison of Czech isolate with Danish isolate 54-15. The only difference between 2 isolates was found in coat protein (CP) gene. Interestingly, the CP of the Czech isolate seems, to be 100 % identical to the one of Sw, while many changes were found in the region encoding TGBp2, TGBp3 and cysteine-rich protein (CRP) for these 2 isolates. The lowest similarity scores were found when comparing the Czech isolate CRP with CRP of Scottish isolates.
DOI: IEB authors: Noemi Čeřovská, Tamara Pečenková