Čeština
EnglishSynthesis, biological, immunological and anticancer properties of a new brassinosteroid ligand
Swaczynová, Jana; Šíša, Miroslav; Hniličková, Jaroslava; Kohout, Ladislav; Strnad, Miroslav
POLISH JOURNAL OF CHEMISTRY 80 [4]: 629-635, 2006
Keywords: Brassinosteroids; phytohormones; antibodies; structure-activity relationship
Abstract: We have developed polyclonal antibodies against the brassinosteroid, 24-epicastasterone. Antiserum against this substance was produced by immunizing rabbits and mice with 24-epicastasterone O-(carboxymethyl)oxime (24-epiCS-CMO) conjugated with bovine-serum albumin (BSA). The use of the ELISA allowed detection over the range of 0.01 500 pmoles. Natural brassinosteroids (BRs) like brassinolide, and 24-epibrassinolide exhibited relatively high cross-reactivities but many other natural BRs were inactive.
DOI:
IEB authors: Miroslav Strnad
POLISH JOURNAL OF CHEMISTRY 80 [4]: 629-635, 2006
Keywords: Brassinosteroids; phytohormones; antibodies; structure-activity relationship
Abstract: We have developed polyclonal antibodies against the brassinosteroid, 24-epicastasterone. Antiserum against this substance was produced by immunizing rabbits and mice with 24-epicastasterone O-(carboxymethyl)oxime (24-epiCS-CMO) conjugated with bovine-serum albumin (BSA). The use of the ELISA allowed detection over the range of 0.01 500 pmoles. Natural brassinosteroids (BRs) like brassinolide, and 24-epibrassinolide exhibited relatively high cross-reactivities but many other natural BRs were inactive.
DOI: