Čeština
EnglishTransgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
Latif M.F.,Tan J., Zhang W., ·Yang W.,·Zhuang T.,· Lu W.,· Qiu Y., Du X., Zhuang X.,· Zhou T., Kundu J.K., Yin J.,Xu K.,
TRANSGENIC RESEARCH 33: 149-157, 2024
Keywords: MicroRNA · Potyvirus · SMV · P1 gene · Transgenic · Virus resistance
Abstract: RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artifcial microRNA amiRNA) can be engineered to specifcally induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efcient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be efective in generating resistance to SMV.
DOI: 10.1007/s11248-024-00388-8 IEB authors: Jiban Kumar
TRANSGENIC RESEARCH 33: 149-157, 2024
Keywords: MicroRNA · Potyvirus · SMV · P1 gene · Transgenic · Virus resistance
Abstract: RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artifcial microRNA amiRNA) can be engineered to specifcally induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efcient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be efective in generating resistance to SMV.
DOI: 10.1007/s11248-024-00388-8 IEB authors: Jiban Kumar