New techniques for the estimation of naturally occurring brassinosteroids

Swaczynová, Jana; Novák, Ondřej; Hauserová, Eva; Fuksová, Květoslava; Šíša, Miroslav; Kohout, Ladislav; Strnad, Miroslav
JOURNAL OF PLANT GROWTH REGULATION 26 [1]: 1-14, 2007

Klíčová slova: antibodies; brassinosteroids; enzyme immunoassay; ELISA
Abstrakt: We have developed enzyme-linked immunosorbent assays (ELISAs) for measuring 24-epicastasterone and related brassinolide analogs, with detection ranges of 0.005 to 50pmoles. Polyclonal antibodies used in these assays were raised against 24-epicastasterone carboxymethyloxime-bovine serum albumin conjugates and were found to have high specificity for 24-epibrassinosteroids.
DOI:
Fulltext: 2007_Swaczynova_J_Plant_Reg... (463.04 kB)
Autoři z ÚEB: Ondřej Novák, Miroslav Strnad